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dc.contributor.authorSalerno-Goncalves, R.
dc.contributor.authorKayastha, D.
dc.contributor.authorFasano, A.
dc.contributor.authorLevine, M.M.
dc.contributor.authorSztein, M.B.
dc.date.accessioned2019-09-14T14:28:33Z
dc.date.available2019-09-14T14:28:33Z
dc.date.issued2019
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85071708929&doi=10.1371%2fjournal.pntd.0007650&partnerID=40&md5=3e86a1af913a6cf2d1ea9697fe7d938e
dc.identifier.urihttp://hdl.handle.net/10713/10836
dc.description.abstractEnteric fevers, caused by the Salmonella enterica serovars Typhi (ST), Paratyphi A (PA) and Paratyphi B (PB), are life-threatening illnesses exhibiting very similar clinical symptoms but with distinct epidemiologies, geographical distributions and susceptibilities to antimicrobial treatment. Nevertheless, the mechanisms by which the host recognizes pathogens with high levels of homology, such as these bacterial serovars, remain poorly understood. Using a three-dimensional organotypic model of the human intestinal mucosa and PA, PB, and ST, we observed significant differences in the secretion patterns of pro-inflammatory cytokines and chemokines elicited by these serovars. These cytokines/chemokines were likely to be co-regulated and influenced the function of epithelial cells, such as the production of IL-8. We also found differing levels of polymorphonuclear leukocyte (PMN) migration among various infection conditions that either included or excluded lymphocytes and macrophages (Mϕ), strongly suggesting feedback mechanisms among these cells. Blocking experiments showed that IL-1β, IL-6, IL-8, TNF-α and CCL3 cytokines were involved in the differential regulation of migration patterns. We conclude that the crosstalk among the lymphocytes, Mϕ, PMN and epithelial cells is cytokine/chemokine-dependent and bacterial-serotype specific, and plays a pivotal role in orchestrating the functional efficiency of the innate cells and migratory characteristics of the leukocytes.en_US
dc.description.urihttps://doi.org/10.1371/journal.pntd.0007650en_US
dc.language.isoen-USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.ispartofPLoS neglected tropical diseases
dc.subject.meshChemokinesen_US
dc.subject.meshCytokinesen_US
dc.subject.meshSalmonella paratyphi Aen_US
dc.subject.meshSalmonella typhien_US
dc.titleCrosstalk between leukocytes triggers differential immune responses against Salmonella enterica serovars Typhi and Paratyphien_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pntd.0007650
dc.identifier.pmid31412039


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