• Login
    View Item 
    •   UMB Digital Archive
    • UMB Open Access Articles
    • UMB Open Access Articles 2019
    • View Item
    •   UMB Digital Archive
    • UMB Open Access Articles
    • UMB Open Access Articles 2019
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of UMB Digital ArchiveCommunitiesPublication DateAuthorsTitlesSubjectsThis CollectionPublication DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    Display statistics

    The bacterial communities of the small intestine and stool in children with short bowel syndrome

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Author
    Zeichner, S.L.
    Mongodin, E.F.
    Hittle, L.
    Date
    2019
    Journal
    PLoS ONE
    Publisher
    Public Library of Science
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://doi.org/10.1371/journal.pone.0215351
    Abstract
    Short bowel syndrome (SBS) presents an increasing problem in pediatrics. SBS often results from surgical resection of necrotic bowel following necrotizing enterocolitis or treatment of anatomic gastrointestinal defects. SBS is associated with significant morbidity and mortality, and creates substantial burdens for patients, families, and the health system. Recent reports have demonstrated that the fecal microbiome of children with SBS is significantly different from healthy control and severe intestinal microbial imbalances is associated with poor growth. We hypothesized that children with SBS and adverse clinical features such as PN dependent, shorter bowel length and lack of ileocecal valve would demonstrate more gut dysbiosis compare with the SBS non-PN dependent. An improved understanding of SBS pathogenesis would enhance management and potentially suggest new interventions. We studied microbial communities of SBS and control non-SBS patients from the jejunum, obtained endoscopically or by ostomy aspiration, and stool. We enrolled SBS patients who did and did not require parenteral nutrition (PN), as a surrogate marker for the seriousness of their disease. We studied the microbiota using high-throughput DNA sequencing of 16S rRNA genes and statistical analyses. We found that microbial diversity was significantly greater in jejunal aspirate than in stool samples in SBS patients, unlike non-SBS patients; that SBS patients receiving enteral feeds had greater diversity, and that SBS patients on PN and enteral feeds had lower differences in diversity in jejunal vs. stool samples. We found a trend toward increased diversity in patients with an intact ileocecal valve, and found that certain taxa were more abundant in the certain sample types, and in SBS patients vs. non-SBS patients. SBS patients have lower microbial diversity, especially patients with more severe disease, patients requiring PN, and those lacking an ileocecal valve. SBS patients, particularly those with more complex characteristics, exhibit differences in their intestinal microbiota. Particular individual taxa were over- and under-represented in patients with more unfavorable disease. While diminished diversity and alterations in microbiota composition are likely consequences of SBS, future efforts aimed at increasing microbial diversity and interventions targeting specific microbiota characteristics might constitute a testable approach to ameliorate some clinical SBS clinical consequences. Copyright 2019 Zeichner et al.
    Keyword
    Intestine, Small--microbiology
    Short Bowel Syndrome
    Pediatrics
    Identifier to cite or link to this item
    https://www.scopus.com/inward/record.uri?eid=2-s2.0-85065911691&doi=10.1371%2fjournal.pone.0215351&partnerID=40&md5=77634ebc6e49a0caa8aa2d606c2ad959; http://hdl.handle.net/10713/10778
    ae974a485f413a2113503eed53cd6c53
    10.1371/journal.pone.0215351
    Scopus Count
    Collections
    UMB Open Access Articles 2019

    entitlement

    Related articles

    • Severe Gut Microbiota Dysbiosis Is Associated With Poor Growth in Patients With Short Bowel Syndrome.
    • Authors: Piper HG, Fan D, Coughlin LA, Ho EX, McDaniel MM, Channabasappa N, Kim J, Kim M, Zhan X, Xie Y, Koh AY
    • Issue date: 2017 Sep
    • The Fecal Microbiome in Pediatric Patients With Short Bowel Syndrome.
    • Authors: Davidovics ZH, Carter BA, Luna RA, Hollister EB, Shulman RJ, Versalovic J
    • Issue date: 2016 Nov
    • Microbial alteration of small bowel stoma effluents and colonic feces in infants with short bowel syndrome.
    • Authors: Zhang T, Wang Y, Yan W, Lu L, Tao Y, Jia J, Cai W
    • Issue date: 2020 Jul
    • A comparison of small bowel and fecal microbiota in children with short bowel syndrome.
    • Authors: Piper HG, Coughlin LA, Nguyen V, Channabasappa N, Koh AY
    • Issue date: 2020 May
    • Fecal microbiota signatures of adult patients with different types of short bowel syndrome.
    • Authors: Huang Y, Guo F, Li Y, Wang J, Li J
    • Issue date: 2017 Dec
    DSpace software (copyright © 2002 - 2021)  DuraSpace
    Quick Guide | Policies | Contact Us | UMB Health Sciences & Human Services Library
    Open Repository is a service operated by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.