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dc.contributor.authorKhan, M.M.
dc.contributor.authorChattagul, S.
dc.contributor.authorTran, B.Q.
dc.date.accessioned2019-09-13T16:42:05Z
dc.date.available2019-09-13T16:42:05Z
dc.date.issued2019
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85065432584&doi=10.1093%2ffemspd%2fftz005&partnerID=40&md5=4abe79cc7b8e9199b31bf0d4068a58a6
dc.identifier.urihttp://hdl.handle.net/10713/10773
dc.description.abstractMelioidosis associated with opportunistic pathogen Burkholderia pseudomallei imparts a huge medical burden in Southeast Asia and Australia. At present there is no available human vaccine that protects against B. pseudomallei infection and antibiotic treatments are limited particularly for drug-resistant strains and bacteria in biofilm forms. Biofilm forming bacteria exhibit phenotypic features drastically different to their planktonic states, often exhibiting a diminished response to antimicrobial therapies. Our earlier work on global profiling of bacterial biofilms using transcriptomics and proteomics revealed transcript-decoupled protein abundance in bacterial biofilms. Here we employed reverse phase liquid chromatography tandem mass spectrometry (LC-MS/MS) to deduce temporal proteomic differences in planktonic and biofilm forms of Burkholderia thailandensis, which is weakly surrogate model of pathogenic B. pseudomallei as sharing a key element in genomic similarity. The proteomic analysis of B. thailandensis in biofilm versus planktonic states revealed that proteome changes support biofilm survival through decreased abundance of metabolic proteins while increased abundance of stress-related proteins. Interestingly, the protein abundance including for the transcription protein TEX, outer periplasmic TolB protein, and the exopolyphosphatase reveal adaption in bacterial biofilms that facilitate antibiotic tolerance through a non-specific mechanism. The present proteomics study of B. thailandensis biofilms provides a global snapshot of protein abundance differences and antimicrobial sensitivities in planktonic and sessile bacteria.en_US
dc.description.urihttps://doi.org/10.1093/femspd/ftz005en_US
dc.language.isoen-USen_US
dc.publisherOxford University Pressen_US
dc.relation.ispartofPathogens and Disease
dc.subjectAntimicrobial toleranceen_US
dc.subjectBiofilmsen_US
dc.subjectLiquid chromatography mass spectrometryen_US
dc.subjectMelioidosisen_US
dc.subjectProteomicsen_US
dc.titleTemporal proteomic profiling reveals changes that support Burkholderia biofilmsen_US
dc.typeArticleen_US
dc.identifier.doi10.1093/femspd/ftz005
dc.identifier.pmid30759239


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