Reciprocal inhibitory glomerular circuits contribute to excitation-inhibition balance in the mouse olfactory bulb
PublisherSociety for Neuroscience
MetadataShow full item record
AbstractThe major inhibitory interneurons in olfactory bulb (OB) glomeruli are periglomerular cells (PGCs) and short axon cells (SACs). PGCs and SACs provide feedforward inhibition to all classes of projection neurons, but inhibition between PGCs and SACs is not well understood. We crossed Cre and GFP transgenic mice and used virally-delivered optogenetic constructs to selectively activate either SACs or GAD65cre-ChR2-positive PGCs while recording from identified GAD65cre-ChR2-positive PGCs or SACs, respectively, to investigate inhibitory interactions between these two interneuron types. We show that GAD65cre-ChR2-positive PGCs robustly inhibit SACs and SACs strongly inhibit PGCs. SACs form the interglomerular circuit, which inhibits PGCs in distant glomeruli. Activation of GAD65cre-ChR2-positive PGCs monosynaptically inhibit mitral cells (MCs), which complements recent findings that SACs directly inhibit MCs. Thus, both classes of glomerular inhibitory neurons inhibit each other, as well as OB output neurons. We further show that olfactory nerve input to one glomerulus engages the interglomerular circuit and inhibits PGCs in distant glomeruli. Sensory activation of the interglomerular circuit directly inhibits output neurons in other glomeruli and by inhibiting intraglomerular PGCs, may potentially disinhibit output neurons in other glomeruli. The nature and context of odorant stimuli may determine whether inhibition or excitation prevails so that odors are represented in part by patterns of active and inactive glomeruli. Copyright 2019 Shao et al.
SponsorsThis work was supported by National Institutes of Health Grants DC010915 and DC005676.
Identifier to cite or link to this itemhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85068196855&doi=10.1523%2fENEURO.0048-19.2019&partnerID=40&md5=f546ccff8e5a83b9eb0d39b1e521124c; http://hdl.handle.net/10713/10740
- The Interglomerular Circuit Potently Inhibits Olfactory Bulb Output Neurons by Both Direct and Indirect Pathways.
- Authors: Liu S, Puche AC, Shipley MT
- Issue date: 2016 Sep 14
- Cholecystokinin selectively activates short axon cells to enhance inhibition of olfactory bulb output neurons.
- Authors: Liu X, Liu S
- Issue date: 2018 Jun
- Serotonin increases synaptic activity in olfactory bulb glomeruli.
- Authors: Brill J, Shao Z, Puche AC, Wachowiak M, Shipley MT
- Issue date: 2016 Mar
- Muscarinic receptors modulate dendrodendritic inhibitory synapses to sculpt glomerular output.
- Authors: Liu S, Shao Z, Puche A, Wachowiak M, Rothermel M, Shipley MT
- Issue date: 2015 Apr 8
- Short-term plasticity in glomerular inhibitory circuits shapes olfactory bulb output.
- Authors: Zhou FW, Shao ZY, Shipley MT, Puche AC
- Issue date: 2020 Mar 1