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dc.contributor.authorTurnquist, C.
dc.contributor.authorBeck, J.A.
dc.contributor.authorHorikawa, I.
dc.date.accessioned2019-09-13T16:42:02Z
dc.date.available2019-09-13T16:42:02Z
dc.date.issued2019
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85063277119&doi=10.1093%2fneuonc%2fnoz001&partnerID=40&md5=44dc537754a6c3116bf1b66329462bfc
dc.identifier.urihttp://hdl.handle.net/10713/10736
dc.description.abstracten_US
dc.description.sponsorshipBackground: Cellular senescence and the senescence-associated secretory phenotype (SASP) may contribute to the development of radiation therapy–associated side effects in the lung and blood vessels by promoting chronic inflammation. In the brain, inflammation contributes to the development of neurologic disease, including Alzheimer’s disease. In this study, we investigated the roles of cellular senescence and Δ133p53, an inhibitory isoform of p53, in radiation-induced brain injury. Methods. Senescent cell types in irradiated human brain were identified with immunohistochemical labeling of senescence-associated proteins p16INK4A and heterochromatin protein Hp1γ in 13 patient cases, including 7 irradiated samples. To investigate the impact of radiation on astrocytes specifically, primary human astrocytes were irradiated and examined for expression of Δ133p53 and induction of SASP. Lentiviral expression of ∆133p53 was performed to investigate its role in regulating radiation-induced cellular senescence and astrocyte-mediated neuroinflammation. Results. Astrocytes expressing p16INK4A and Hp1γ were identified in all irradiated tissues, were increased in number in irradiated compared with untreated cancer patient tissues, and had higher labeling intensity in irradiated tissues compared with age-matched controls. Human astrocytes irradiated in vitro also experience induction of cellular senescence, have diminished Δ133p53, and adopt a neurotoxic phenotype as demonstrated by increased senescence-associated beta-galactosidase activity, p16INK4A, and interleukin (IL)-6. In human astrocytes, Δ133p53 inhibits radiation-induced senescence, promotes DNA double-strand break repair, and prevents astrocyte-mediated neuroinflammation and neurotoxicity. Conclusions. Restoring expression of the endogenous p53 isoform, ∆133p53, protects astrocytes from radiationinduced senescence, promotes DNA repair, and inhibits astrocyte-mediated neuroinflammation.en_US
dc.description.urihttps://doi.org/10.1093/neuonc/noz001en_US
dc.language.isoen-USen_US
dc.publisherOxford University Pressen_US
dc.relation.ispartofNeuro-Oncology
dc.subjectastrocytesen_US
dc.subjectIL-6en_US
dc.subjectp53 isoformen_US
dc.subjectradiation-induced brain injuryen_US
dc.subjectsenescenceen_US
dc.titleRadiation-induced astrocyte senescence is rescued by Δ133p53en_US
dc.typeArticleen_US
dc.identifier.doi10.1093/neuonc/noz001
dc.identifier.pmid30615147


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