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    Properties of individual hippocampal synapses influencing NMDA-receptor activation by spontaneous neurotransmission

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    Author
    Metzbower, S.R.
    Joo, Y.
    Benavides, D.R.
    Date
    2019
    Journal
    eNeuro
    Publisher
    Society for Neuroscience
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://doi.org/10.1523/ENEURO.0419-18.2019
    Abstract
    NMDA receptor (NMDAR) activation is critical for maintenance and modification of synapse strength. Specifically, NMDAR activation by spontaneous glutamate release has been shown to mediate some forms of synaptic plasticity as well as synaptic development. Interestingly, there is evidence that within individual synapses each release mode may be segregated such that postsynaptically there are distinct pools of responsive receptors. To examine potential regulators of NMDAR activation because of spontaneous glutamate release in cultured hippocampal neurons, we used GCaMP6f imaging at single synapses in concert with confocal and super-resolution imaging. Using these single-spine approaches, we found that Ca2+ entry activated by spontaneous release tends to be carried by GluN2B-NMDARs. Additionally, the amount of NMDAR activation varies greatly both between synapses and within synapses, and is unrelated to spine and synapse size, but does correlate loosely with synapse distance from the soma. Despite the critical role of spontaneous activation of NMDARs in maintaining synaptic function, their activation seems to be controlled factors other than synapse size or synapse distance from the soma. It is most likely that NMDAR activation by spontaneous release influenced variability in subsynaptic receptor position, release site position, vesicle content, and channel properties. Therefore, spontaneous activation of NMDARs appears to be regulated distinctly from other receptor types, notably AMPARs, within individual synapses. Copyright 2019 Metzbower et al.
    Keyword
    Calcium imaging
    GCaMP
    Postsynaptic density
    STORM
    Super-resolution
    Identifier to cite or link to this item
    https://www.scopus.com/inward/record.uri?eid=2-s2.0-85067268505&doi=10.1523%2fENEURO.0419-18.2019&partnerID=40&md5=1d9507fd5363c33049e010a63577098f; http://hdl.handle.net/10713/10729
    ae974a485f413a2113503eed53cd6c53
    10.1523/ENEURO.0419-18.2019
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