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    Oxytocin can regulate myometrial smooth muscle excitability by inhibiting the Na + -activated K + channel, Slo2.1

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    Author
    Ferreira, J.J.
    Butler, A.
    Stewart, R.
    Date
    2019
    Journal
    Journal of Physiology
    Publisher
    Blackwell Publishing Ltd
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://doi.org/10.1113/JP276806
    Abstract
    Key points: At the end of pregnancy, the uterus transitions from a quiescent state to a highly contractile state. This transition requires that the uterine (myometrial) smooth muscle cells increase their excitability, although how this occurs is not fully understood. We identified SLO2.1, a potassium channel previously unknown in uterine smooth muscle, as a potential significant contributor to the electrical excitability of myometrial smooth muscle cells. We found that activity of the SLO2.1 channel is negatively regulated by oxytocin via G?q-protein-coupled receptor activation of protein kinase C. This results in depolarization of the uterine smooth muscle cells and calcium entry, which may contribute to uterine contraction. These findings provide novel insights into a previously unknown mechanism by which oxytocin may act to modulate myometrial smooth muscle cell excitability. Our findings also reveal a new potential pharmacological target for modulating uterine excitability. Abstract: During pregnancy, the uterus transitions from a quiescent state to a more excitable contractile state. This is considered to be at least partly a result of changes in the myometrial smooth muscle cell (MSMC) resting membrane potential. However, the ion channels controlling the myometrial resting membrane potential and the mechanism of transition to a more excitable state have not been fully clarified. In the present study, we show that the sodium-activated, high-conductance, potassium leak channel, SLO2.1, is expressed and active at the resting membrane potential in MSMCs. Additionally, we report that SLO2.1 is inhibited by oxytocin binding to the oxytocin receptor. Inhibition of SLO2.1 leads to membrane depolarization and activation of voltage-dependent calcium channels, resulting in calcium influx. The results of the present study reveal that oxytocin may modulate MSMC electrical activity by inhibiting SLO2.1 potassium channels. Copyright 2018 The Authors.
    Sponsors
    This work was supported by Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) R01 HD088097 to CMS and SKE as well as (NIGMS and NIMH) R01 GM114694 and R21MH107955 to LS, respectively.
    Keyword
    Myometrium
    Oxytocin
    SLO2.1 Potassium channels
    Smooth muscle
    Identifier to cite or link to this item
    https://www.scopus.com/inward/record.uri?eid=2-s2.0-85056821056&doi=10.1113%2fJP276806&partnerID=40&md5=d7a5338fb36cb8709ba4deac5c65b502; http://hdl.handle.net/10713/10707
    ae974a485f413a2113503eed53cd6c53
    10.1113/JP276806
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    UMB Open Access Articles 2019

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