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    Label-free surface-sensitive photonic microscopy with high spatial resolution using azimuthal rotation illumination

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    Author
    Kuai, Y.
    Chen, J.
    Tang, X.
    Date
    2019
    Journal
    Science Advances
    Publisher
    American Association for the Advancement of Science
    Type
    Article
    
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    Show full item record
    See at
    https://doi.org/10.1126/sciadv.aav5335
    Abstract
    Surface plasmon resonance microscopy (SPRM) with single-direction illumination is a powerful platform for biomedical imaging because of its wide-field, label-free, and high-surface-sensitivity imaging capabilities. However, two disadvantages prevent wider use of SPRM. The first is its poor spatial resolution that can be as large as several micrometers. The second is that SPRM requires use of metal films as sample substrates; this introduces working wavelength limitations. In addition, cell culture growth on metal films is not as universally available as growth on dielectric substrates. Here we show that use of azimuthal rotation illumination allows SPRM spatial resolution to be enhanced by up to an order of magnitude. The metal film can also be replaced by a dielectric multilayer and then a different label-free surface-sensitive photonic microscopy is developed, which has more choices in terms of the working wavelength, polarization, and imaging section, and will bring opportunities for applications in biology. Copyright Copyright 2019 The Authors, some rights reserved;
    Keyword
    surface plasmon resonance microscopy
    SPRM
    Surface Plasmon Resonance--methods
    Identifier to cite or link to this item
    https://www.scopus.com/inward/record.uri?eid=2-s2.0-85064040434&doi=10.1126%2fsciadv.aav5335&partnerID=40&md5=13647923bdc798f0223086330c05e14c; http://hdl.handle.net/10713/10673
    ae974a485f413a2113503eed53cd6c53
    10.1126/sciadv.aav5335
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    UMB Open Access Articles 2019

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