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dc.contributor.authorFiske, C.T.
dc.contributor.authorBlackman, A.
dc.contributor.authorMaruri, F.
dc.date.accessioned2019-09-13T16:41:57Z
dc.date.available2019-09-13T16:41:57Z
dc.date.issued2019
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85065123565&doi=10.1186%2fs12879-019-3958-7&partnerID=40&md5=d44fc43999c442da19576b5dd485af28
dc.identifier.urihttp://hdl.handle.net/10713/10663
dc.description.abstractBackground: Independent of HIV infection, extrapulmonary TB (EPTB) risk is increased in women, persons of black race or foreign birth, and by genetic variants in vitamin D receptor (VDR), interleukin-1 beta (IL-1β), and toll-like receptor (TLR)-2; functional correlates are unclear. We evaluated macrophage expression of VDR, TLR2, cathelicidin, and TNF-α, and production of IL-1β in HIV-seronegative persons with previous EPTB, previous pulmonary TB, latent M. tuberculosis infection, and uninfected TB contacts. Persons with previous pleural TB were excluded due to enhanced immune responses at the site of disease. Methods: Macrophages were stimulated with TLR-2 agonist M. tuberculosis lipoprotein (LpqH), live and gamma-irradiated M. tuberculosis. Results: M. tuberculosis – infected macrophages from persons with previous EPTB had increased VDR expression (29.17 relative value unit increase in median expression vs. uninfected contacts, after adjusting for foreign-born status; P = 0.02). Macrophages from persons with previous EPTB had a 38.88 μg/mL increase in median IL-1β production after stimulation with LpqH compared to uninfected contacts (P = 0.01); the effect was similar (44.99 μg/mL) but not statistically significant after controlling for foreign-born status. Median 25-hydroxyvitamin D levels were low but not significantly different between groups. Conclusions: There was increased macrophage expression of VDR after stimulation with live M. tuberculosis in persons with previous extrapulmonary TB. If post-treatment VDR expression reflects expression prior to disease, it may identify persons at risk for extrapulmonary TB. Copyright 2019 The Author(s).en_US
dc.description.sponsorshipNational Institutes of Health (K23AI091692 (CTF), K24AI065298 (AB, TRS), Potts Memorial Foundation.en_US
dc.description.urihttps://doi.org/10.1186/s12879-019-3958-7en_US
dc.language.isoen-USen_US
dc.publisherBioMed Central Ltd.en_US
dc.relation.ispartofBMC Infectious Diseases
dc.subjectExtrapulmonary tuberculosisen_US
dc.subjectInterleukin-1 betaen_US
dc.subjectM. Tuberculosisen_US
dc.subjectToll-like receptor 2en_US
dc.subjectVitamin D receptoren_US
dc.titleIncreased vitamin D receptor expression from macrophages after stimulation with M. tuberculosis among persons who have recovered from extrapulmonary tuberculosisen_US
dc.typeArticleen_US
dc.identifier.doi10.1186/s12879-019-3958-7
dc.identifier.pmid31039752


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