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dc.contributor.authorHudgens, J.W.
dc.contributor.authorGallagher, E.S.
dc.contributor.authorKarageorgos, I.
dc.date.accessioned2019-09-13T16:41:56Z
dc.date.available2019-09-13T16:41:56Z
dc.date.issued2019
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85066796565&doi=10.6028%2fjres.124.009&partnerID=40&md5=2b0fd8c1e350228597a2a9e3749262d7
dc.identifier.urihttp://hdl.handle.net/10713/10659
dc.description.abstractThe spreadsheet file reported herein provides centroid data, descriptive of deuterium uptake, for the Fab Fragment of NISTmAb (PDB: 5K8A) reference material, as measured by the bottom-up hydrogen-deuterium exchange mass spectrometry (HDX-MS) method. The protein sample was incubated in deuterium-rich solutions under uniform pH and salt concentrations between 3.6 o C and 25.4 o C for seven intervals ranging over (0 to 14,400) s plus a ∞pseudo s control. The deuterium content of peptic peptide fragments were measured by mass spectrometry. [1-3] These data were reported by fifteen laboratories, which conducted the measurements using orbitrap and QTOF mass spectrometers. The cohort reported ≈ 78,900 centroids for 430 proteolytic peptide sequences of the heavy and light chains of NISTmAb, providing nearly 100 % coverage. In addition, some groups reported ≈ 10,900 centroid measurements for 77 peptide sequences of the Fc fragment. The instrumentation and physical and chemical conditions under which these data were acquired are documented.en_US
dc.description.urihttps://doi.org/10.6028/jres.124.009en_US
dc.language.isoen-USen_US
dc.publisherNational Institute of Standards and Technologyen_US
dc.relation.ispartofJournal of Research of the National Institute of Standards and Technology
dc.subjectCentroiden_US
dc.subjectHydrogen-deuterium exchangeen_US
dc.subjectInterlaboratory comparisonen_US
dc.subjectMass spectrometryen_US
dc.subjectPeptideen_US
dc.subjectPrecisionen_US
dc.subjectProteomicsen_US
dc.subjectReference materialen_US
dc.subjectRepeatabilityen_US
dc.subjectReproducibilityen_US
dc.titleHydrogen-deuterium exchange mass spectrometry (HDX-MS) centroid data measured between 3.6 oC and 25.4 OC for the Fab fragment of NiStmaben_US
dc.typeArticleen_US
dc.identifier.doi10.6028/jres.124.009


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