Hydrogen-deuterium exchange mass spectrometry (HDX-MS) centroid data measured between 3.6 oC and 25.4 OC for the Fab fragment of NiStmab
Date
2019Journal
Journal of Research of the National Institute of Standards and TechnologyPublisher
National Institute of Standards and TechnologyType
Article
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The spreadsheet file reported herein provides centroid data, descriptive of deuterium uptake, for the Fab Fragment of NISTmAb (PDB: 5K8A) reference material, as measured by the bottom-up hydrogen-deuterium exchange mass spectrometry (HDX-MS) method. The protein sample was incubated in deuterium-rich solutions under uniform pH and salt concentrations between 3.6 o C and 25.4 o C for seven intervals ranging over (0 to 14,400) s plus a ∞pseudo s control. The deuterium content of peptic peptide fragments were measured by mass spectrometry. [1-3] These data were reported by fifteen laboratories, which conducted the measurements using orbitrap and QTOF mass spectrometers. The cohort reported ≈ 78,900 centroids for 430 proteolytic peptide sequences of the heavy and light chains of NISTmAb, providing nearly 100 % coverage. In addition, some groups reported ≈ 10,900 centroid measurements for 77 peptide sequences of the Fc fragment. The instrumentation and physical and chemical conditions under which these data were acquired are documented.Keyword
CentroidHydrogen-deuterium exchange
Interlaboratory comparison
Mass spectrometry
Peptide
Precision
Proteomics
Reference material
Repeatability
Reproducibility
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85066796565&doi=10.6028%2fjres.124.009&partnerID=40&md5=2b0fd8c1e350228597a2a9e3749262d7; http://hdl.handle.net/10713/10659ae974a485f413a2113503eed53cd6c53
10.6028/jres.124.009