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    Hydrogen-deuterium exchange mass spectrometry (HDX-MS) centroid data measured between 3.6 oC and 25.4 OC for the Fab fragment of NiStmab

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    Author
    Hudgens, J.W.
    Gallagher, E.S.
    Karageorgos, I.
    Date
    2019
    Journal
    Journal of Research of the National Institute of Standards and Technology
    Publisher
    National Institute of Standards and Technology
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://doi.org/10.6028/jres.124.009
    Abstract
    The spreadsheet file reported herein provides centroid data, descriptive of deuterium uptake, for the Fab Fragment of NISTmAb (PDB: 5K8A) reference material, as measured by the bottom-up hydrogen-deuterium exchange mass spectrometry (HDX-MS) method. The protein sample was incubated in deuterium-rich solutions under uniform pH and salt concentrations between 3.6 o C and 25.4 o C for seven intervals ranging over (0 to 14,400) s plus a ∞pseudo s control. The deuterium content of peptic peptide fragments were measured by mass spectrometry. [1-3] These data were reported by fifteen laboratories, which conducted the measurements using orbitrap and QTOF mass spectrometers. The cohort reported ≈ 78,900 centroids for 430 proteolytic peptide sequences of the heavy and light chains of NISTmAb, providing nearly 100 % coverage. In addition, some groups reported ≈ 10,900 centroid measurements for 77 peptide sequences of the Fc fragment. The instrumentation and physical and chemical conditions under which these data were acquired are documented.
    Keyword
    Centroid
    Hydrogen-deuterium exchange
    Interlaboratory comparison
    Mass spectrometry
    Peptide
    Precision
    Proteomics
    Reference material
    Repeatability
    Reproducibility
    Identifier to cite or link to this item
    https://www.scopus.com/inward/record.uri?eid=2-s2.0-85066796565&doi=10.6028%2fjres.124.009&partnerID=40&md5=2b0fd8c1e350228597a2a9e3749262d7; http://hdl.handle.net/10713/10659
    ae974a485f413a2113503eed53cd6c53
    10.6028/jres.124.009
    Scopus Count
    Collections
    UMB Open Access Articles 2019

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