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dc.contributor.authorAnastasiadis, P.
dc.contributor.authorMohammadabadi, A.
dc.contributor.authorFishman, M.J.
dc.date.accessioned2019-09-13T14:49:32Z
dc.date.available2019-09-13T14:49:32Z
dc.date.issued2019
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85063743176&doi=10.1186%2fs12938-019-0656-z&partnerID=40&md5=d1f119c42a9695d469272798c3139141
dc.identifier.urihttp://hdl.handle.net/10713/10599
dc.description.abstractBackground: The clinical applications of transcranial focused ultrasound continue to expand and include ablation as well as drug delivery applications in the brain, where treatments are typically guided by MRI. Although MRI-guided focused ultrasound systems are also preferred for many preclinical investigations, they are expensive to purchase and operate, and require the presence of a nearby imaging center. For many basic mechanistic studies, however, MRI is not required. The purpose of this study was to design, construct, characterize and evaluate a portable, custom, laser-guided focused ultrasound system for noninvasive, transcranial treatments in small rodents. Methods: The system comprised an off-the-shelf focused ultrasound transducer and amplifier, with a custom cone fabricated for direct coupling of the transducer to the head region. A laser-guidance apparatus was constructed with a 3D stage for accurate positioning to 1 mm. Pressure field simulations were performed to demonstrate the effects of the coupling cone and the sealing membrane, as well as for determining the location of the focus and acoustic transmission across rat skulls over a range of sizes. Hydrophone measurements and exposures in hydrogels were used to assess the accuracy of the simulations. In vivo treatments were performed in rodents for opening the blood-brain barrier and to assess the performance and accuracy of the system. The effects of varying the acoustic pressure, microbubble dose and animal size were evaluated in terms of efficacy and safety of the treatments. Results: The simulation results were validated by the hydrophone measurements and exposures in the hydrogels. The in vivo treatments demonstrated the ability of the system to open the blood-brain barrier. A higher acoustic pressure was required in larger-sized animals, as predicted by the simulations and transmission measurements. In a particular sized animal, the degree of blood-brain barrier opening, and the safety of the treatments were directly associated with the microbubble dose. Conclusion: The focused ultrasound system that was developed was found to be a cost-effective alternative to MRI-guided systems as an investigational device that is capable of accurately providing noninvasive, transcranial treatments in rodents. Copyright 2019 The Author(s).en_US
dc.description.urihttps://doi.org/10.1186/s12938-019-0656-zen_US
dc.language.isoen-USen_US
dc.publisherBioMed Central Ltd.en_US
dc.relation.ispartofBioMedical Engineering Online
dc.subjectAcoustic transmissionen_US
dc.subjectBlood-brain barrieren_US
dc.subjectFocused ultrasounden_US
dc.subjectHydrogel phantomsen_US
dc.subjectHydrophone measurementsen_US
dc.subjectLaser targetingen_US
dc.subjectMicrobubblesen_US
dc.subjectMicrohemorrhageen_US
dc.subjectSimulationsen_US
dc.titleDesign, characterization and evaluation of a laser-guided focused ultrasound system for preclinical investigationsen_US
dc.typeArticleen_US
dc.identifier.doi10.1186/s12938-019-0656-z
dc.identifier.pmid30922312


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