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dc.contributor.authorMontero, D.A.
dc.contributor.authorCanto, F.D.
dc.contributor.authorVelasco, J.
dc.date.accessioned2019-09-13T14:49:31Z
dc.date.available2019-09-13T14:49:31Z
dc.date.issued2019
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85064118989&doi=10.1080%2f22221751.2019.1595985&partnerID=40&md5=7dbf75def8cb0b7b44f589d8c7409752
dc.identifier.urihttp://hdl.handle.net/10713/10594
dc.description.abstractShiga toxin-producing Escherichia coli (STEC) are foodborne pathogens causing severe gastroenteritis, which may lead to hemolytic uremic syndrome. The Locus of Enterocyte Effacement (LEE), a Pathogenicity Island (PAI), is a major determinant of intestinal epithelium attachment of a group of STEC strains; however, the virulence repertoire of STEC strains lacking LEE, has not been fully characterized. The incidence of LEE-negative STEC strains has increased in several countries, highlighting the relevance of their study. In order to gain insights into the basis for the emergence of LEE-negative STEC strains, we performed a large-scale genomic analysis of 367 strains isolated worldwide from humans, animals, food and the environment. We identified uncharacterized genomic islands, including two PAIs and one Integrative Conjugative Element. Additionally, the Locus of Adhesion and Autoaggregation (LAA) was the most prevalent PAI among LEE-negative strains and we found that it contributes to colonization of the mice intestine. Our comprehensive and rigorous comparative genomic and phylogenetic analyses suggest that the accumulative acquisition of PAIs has played an important, but currently unappreciated role, in the evolution of virulence in these strains. This study provides new knowledge on the pathogenicity of LEE-negative STEC strains and identifies molecular markers for their epidemiological surveillance. Copyright 2019, Copyright 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd.en_US
dc.description.urihttps://doi.org/10.1080/22221751.2019.1595985en_US
dc.language.isoen-USen_US
dc.publisherTaylor and Francis Ltd.en_US
dc.relation.ispartofEmerging Microbes and Infections
dc.subjectcomparative genomicsen_US
dc.subjectIntegrative Conjugative Elementen_US
dc.subjectLEE-negative STECen_US
dc.subjectLocus of Adhesion and Autoaggregationen_US
dc.subjectPathogenicity Islanden_US
dc.titleCumulative acquisition of pathogenicity islands has shaped virulence potential and contributed to the emergence of LEE-negative Shiga toxin-producing Escherichia coli strainsen_US
dc.typeArticleen_US
dc.identifier.doi10.1080/22221751.2019.1595985
dc.identifier.pmid30924410


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