Show simple item record

dc.contributor.authorIzumi, S.
dc.contributor.authorOtsuru, S.
dc.contributor.authorAdachi, N.
dc.date.accessioned2019-09-13T14:49:31Z
dc.date.available2019-09-13T14:49:31Z
dc.date.issued2019
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85063263115&doi=10.1371%2fjournal.pone.0213912&partnerID=40&md5=a30b80f3fe0d7b53bcab39165afc18f8
dc.identifier.urihttp://hdl.handle.net/10713/10584
dc.description.abstractGlucose metabolism is altered in injured and healing tendons. However, the mechanism by which the glucose metabolism is involved in the pathogenesis of tendon healing process remains unclear. Injured tendons do not completely heal, and often induce fibrous scar and chondroid lesion. Because previous studies have shown that tendon progenitors play roles in tendon repair, we asked whether connective tissue progenitors appearing in injured tendons alter glucose metabolism during tendon healing process. We isolated connective tissue progenitors from the human injured tendons, obtained at the time of primary surgical repair of rupture or laceration. We first characterized the change in glucose metabolism by metabolomics analysis using [1,2- 13 C]-glucose using the cells isolated from the lacerated flexor tendon. The flux of glucose to the glycolysis pathway was increased in the connective tissue progenitors when they proceeded toward tenogenic and chondrogenic differentiation. The influx of glucose to the tricarboxylic acid (TCA) cycle and biosynthesis of amino acids from the intermediates of the TCA cycle were strongly stimulated toward chondrogenic differentiation. When we treated the cultures with 2-deoxy-D-glucose (2DG), an inhibitor of glycolysis, 2DG inhibited chondrogenesis as characterized by accumulation of mucopolysaccharides and expression of AGGRECAN. Interestingly, 2DG strongly stimulated expression of tenogenic transcription factor genes, SCLERAXIS and MOHAWK under both chondrogenic and tenogenic differentiation conditions. The findings suggest that control of glucose metabolism is beneficial for tenogenic differentiation of connective tissue progenitors. Copyright 2019 Izumi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en_US
dc.description.urihttps://doi.org/10.1371/journal.pone.0213912en_US
dc.language.isoen-USen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.ispartofPLoS ONE
dc.subjectconnective tissue progenitorsen_US
dc.subjecttenogenic differentiationen_US
dc.subject.meshTendon Injuriesen_US
dc.subject.meshGlucose Metabolism Disordersen_US
dc.subject.meshDeoxyglucoseen_US
dc.titleControl of glucose metabolism is important in tenogenic differentiation of progenitors derived from human injured tendonsen_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pone.0213912
dc.identifier.pmid30883580


This item appears in the following Collection(s)

Show simple item record