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    A comparison of Lyse-It to other cellular sample preparation, bacterial lysing, and DNA fragmentation technologies

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    Author
    Santaus, T.M.
    Li, S.
    Saha, L.
    Chen, W.H.
    Bhagat, S.
    Stine, O. Colin
    Date
    2019
    Journal
    PLoS ONE
    Publisher
    Public Library of Science
    Type
    Article
    
    Metadata
    Show full item record
    See at
    https://www.doi.org/10.1371/journal.pone.0220102
    Abstract
    The ability for safe and rapid pathogenic sample transportation and subsequent detection is an increasing challenge throughout the world. Herein, we describe and use bead-beating, vortex, sonication, 903 protein saver cards, and Lyse-It methods, aiming to inactivate Gram-positive and -negative bacteria with subsequent genome DNA (quantitative Polymerase Chain Reaction) qPCR detection. The basic concepts behind the four chosen technologies is their versatility, cost, and ease of use in developed and underdeveloped countries. The four methods target the testing of bacterial resilience, cellular extraction from general and complex media and subsequent DNA extraction for qPCR detection and amplification. These results demonstrate that conventional high temperature heating, 903 protein saver cards, and Lyse-It are all viable options for inactivating bacterial growth for safe shipping. Additionally, Lyse-It was found to be particularly useful as this technology can inactivate bacteria, extract cells from 903 protein saver cards, lyse bacterial cells, and additionally keep genomic DNA viable for qPCR detection. Copyright: Copyright 2019 Santaus et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
    Keyword
    pathogenic sample transportation
    Lyse-It
    Identifier to cite or link to this item
    https://www.scopus.com/inward/record.uri?eid=2-s2.0-85069856561&doi=10.1371%2fjournal.pone.0220102&partnerID=40&md5=b47bd92b559bcff127e4578e7c8fb581; http://hdl.handle.net/10713/10345
    ae974a485f413a2113503eed53cd6c53
    10.1371/journal.pone.0220102
    Scopus Count
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    UMB Open Access Articles 2019

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