Sexual dimorphism of mitochondrial function in the hypoxic Guinea pig placenta
Date
2019Journal
Biology of ReproductionPublisher
Oxford University PressType
article
Metadata
Show full item recordAbstract
Placental hypoxia can stimulate oxidative stress and mitochondrial dysfunction reducing placental efficiency and inducing fetal growth restriction (FGR). We hypothesized that chronic hypoxia inhibits mitochondrial function in the placenta as an underlying cause of cellular mechanisms contributing to FGR. Pregnant guinea pigs were exposed to either normoxia (NMX) or hypoxia (HPX; 10.5% O2) at 25 day gestation until term (65 day). Guinea pigs were anesthetized, and fetuses and placentas were excised at either mid (40 day) or late gestation (64 day), weighed, and placental tissue stored at −80°C until assayed. Mitochondrial DNA content, protein expression of respiratory Complexes I-V, and nitration and activity rates of Complexes I and IV were measured in NMX and HPX male (N = 6 in each treatment) and female (N = 6 in each treatment) placentas. Mitochondrial density was not altered by HPX in either mid- or late-term placentas. In mid gestation, HPX slightly increased expression of Complexes I-III and V in male placentas only, but had no effect on either Complex I or IV activity rates or nitrotyrosine expression. In late gestation, HPX significantly decreased CI/CIV activity rates and increased CI/CIV nitration in male but not female placentas exhibiting a sexual dimorphism. Complex I-V expression was reduced from mid to late gestation in both male and female placentas regardless of treatment. We conclude that chronic HPX decreases mitochondrial function by inhibiting Complex I/IV activity via increased peroxynitrite in a sex-related manner. Further, there may be a progressive decrease in energy metabolism of placental cell types with gestation that increases the vulnerability of placental function to intrauterine stress. Copyright The Author(s) 2018.Identifier to cite or link to this item
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85058812898&doi=10.1093%2fbiolre%2fioy167&partnerID=40&md5=02297ca54d025ca37f8420bb4024fbcd; http://hdl.handle.net/10713/10209ae974a485f413a2113503eed53cd6c53
10.1093/biolre/ioy167
Scopus Count
Collections
Related articles
- Sex-Selective Increase of IGF-2 Expression in the Hypoxic Guinea Pig Placenta of Growth-Restricted Fetuses.
- Authors: Elsamadicy EA, Thompson LP
- Issue date: 2022 Oct
- Prenatal hypoxia impairs cardiac mitochondrial and ventricular function in guinea pig offspring in a sex-related manner.
- Authors: Thompson LP, Chen L, Polster BM, Pinkas G, Song H
- Issue date: 2018 Dec 1
- Chronic Hypoxia Inhibits Respiratory Complex IV Activity and Disrupts Mitochondrial Dynamics in the Fetal Guinea Pig Forebrain.
- Authors: Quebedeaux TM, Song H, Giwa-Otusajo J, Thompson LP
- Issue date: 2022 Jan
- Chronic hypoxia alters cardiac mitochondrial complex protein expression and activity in fetal guinea pigs in a sex-selective manner.
- Authors: Song H, Polster BM, Thompson LP
- Issue date: 2021 Dec 1
- Placental Hypoxia During Early Pregnancy Causes Maternal Hypertension and Placental Insufficiency in the Hypoxic Guinea Pig Model.
- Authors: Thompson LP, Pence L, Pinkas G, Song H, Telugu BP
- Issue date: 2016 Dec