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dc.contributor.authorFricker, A.M.
dc.contributor.authorPodlesny, D.
dc.contributor.authorFricke, W.F.
dc.date.accessioned2019-08-05T17:00:29Z
dc.date.available2019-08-05T17:00:29Z
dc.date.issued2019
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85064011322&doi=10.1016%2fj.jare.2019.03.006&partnerID=40&md5=2d962864f37f351889f6d089ed10038d
dc.identifier.urihttp://hdl.handle.net/10713/10203
dc.description.abstractMicrobiome research has transformed the scientific landscape, as reflected by the exponential increase in microbiome-related publications from many different disciplines. Host-associated microbial communities play a role for almost all aspects of human, animal and plant biology and health. Consequently, there are tremendous expectations for the development of new clinical, agricultural and biotechnological applications of microbiome research. However, the field continues to be largely shaped by descriptive studies, the mechanistic understanding of microbiome functions for their hosts remains fragmentary, and direct applications of microbiome research are lacking. The aim of this review is therefore to provide a general introduction to the technical opportunities and challenges of microbiome research, as well as to make experimental and bioinformatic recommendations, i.e. (i) to avoid, reduce and assess the confounding effects of sample storage, nucleic acid isolation and microbial contamination; (ii) to minimize non-microbial contributions in host-associated microbiome samples; (iii) to sharpen the focus on physiologically relevant microbiome features by distinguishing signals from metabolically active and inactive or dead microbes and by adopting quantitative methods; and (iv) to enforce open data and protocol policies in order increase the transparency, reproducibility and credibility of the field.en_US
dc.description.sponsorshipDaniel Podlesny is supported by funds from the German Research Foundation (DFG) under project number 316130265 .en_US
dc.description.urihttps://doi.org/10.1016/j.jare.2019.03.006en_US
dc.language.isoen-USen_US
dc.publisherElsevier B.V.en_US
dc.relation.ispartofJournal of Advanced Research
dc.subjectActive microbiotaen_US
dc.subjectAmplicon sequence varianten_US
dc.subjectContaminationen_US
dc.subjectMicrobiomeen_US
dc.subjectOpen dataen_US
dc.subjectQuantitative profilingen_US
dc.titleWhat is new and relevant for sequencing-based microbiome research? A mini-reviewen_US
dc.typearticleen_US
dc.identifier.doi10.1016/j.jare.2019.03.006


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