Imaging stem cell distribution, growth, migration, and differentiation in 3-D scaffolds for bone tissue engineering using mesoscopic fluorescence tomography
Date
2018Journal
Biotechnology and BioengineeringPublisher
John Wiley and Sons Inc.Type
Article
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Regenerative medicine has emerged as an important discipline that aims to repair injury or replace damaged tissues or organs by introducing living cells or functioning tissues. Successful regenerative medicine strategies will likely depend upon a simultaneous optimization strategy for the design of biomaterials, cell-seeding methods, cell-biomaterial interactions and molecular signaling within the engineered tissues. It remains a challenge to image three-dimensional (3-D) structures and functions of the cell-seeded scaffold in mesoscopic scale (>2~3 mm). In this study, we utilized angled fluorescence laminar optical tomography (aFLOT), which allows depth-resolved molecular characterization of engineered tissues in 3-D to investigate cell viability, migration and bone mineralization within bone tissue engineering scaffolds in situ. Copyright 2017 Wiley Periodicals, Inc.Sponsors
National Institutes of Health, Grant number: R01 EB014946Keyword
3-D cell printingbone mineralization
bone tissue scaffold
cell migration
mesoscopic fluorescence tomography
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https://www.scopus.com/inward/record.uri?eid=2-s2.0-85035057526&doi=10.1002%2fbit.26452&partnerID=40&md5=e2f46d5761403441fabab98e71e6c197; http://hdl.handle.net/10713/10148ae974a485f413a2113503eed53cd6c53
10.1002/bit.26452
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