Imaging stem cell distribution, growth, migration, and differentiation in 3-D scaffolds for bone tissue engineering using mesoscopic fluorescence tomography
JournalBiotechnology and Bioengineering
PublisherJohn Wiley and Sons Inc.
MetadataShow full item record
AbstractRegenerative medicine has emerged as an important discipline that aims to repair injury or replace damaged tissues or organs by introducing living cells or functioning tissues. Successful regenerative medicine strategies will likely depend upon a simultaneous optimization strategy for the design of biomaterials, cell-seeding methods, cell-biomaterial interactions and molecular signaling within the engineered tissues. It remains a challenge to image three-dimensional (3-D) structures and functions of the cell-seeded scaffold in mesoscopic scale (>2~3 mm). In this study, we utilized angled fluorescence laminar optical tomography (aFLOT), which allows depth-resolved molecular characterization of engineered tissues in 3-D to investigate cell viability, migration and bone mineralization within bone tissue engineering scaffolds in situ. Copyright 2017 Wiley Periodicals, Inc.
SponsorsNational Institutes of Health, Grant number: R01 EB014946
Keyword3-D cell printing
bone tissue scaffold
mesoscopic fluorescence tomography
Identifier to cite or link to this itemhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85035057526&doi=10.1002%2fbit.26452&partnerID=40&md5=e2f46d5761403441fabab98e71e6c197; http://hdl.handle.net/10713/10148