Anti-bacterial and microecosystem-regulating effects of dental implant coated with dimethylaminododecyl methacrylate
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AbstractThe effects of dimethylaminododecyl methacrylate (DMADDM) modified titanium implants on bacterial activity and microbial ecosystem of saliva-derived biofilm were investigated for the first time. Titanium discs were coated with DMADDM solutions at mass fractions of 0 mg/mL (control), 1, 5 and 10 mg/mL, respectively. Biomass accumulation and metabolic activity of biofilms were tested using crystal violet assay and MTT (3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. 16S rRNA gene sequencing was performed to measure the microbial community. Live/dead staining and scanning electron microscopy (SEM) were used to value the structure of biofilm. The results showed that the higher mass fraction of DMADDM the coating solution had, the significantly lower the values of metabolic activity and accumulated biofilms got, as well as fewer live cells and less extracellular matrix. Moreover, 5 mg/mL of DMADDM was the most effective concentration, as well as 10 mg/mL. In microecosystem-regulation, the DMADDM modified titanium implant decreased the relative abundance of Neisseria and Actinomyces and increased the relative abundance of Lactobacillus, a probiotic for peri-implant diseases. In conclusion, via inhibiting growth and regulating microecosystem of biofilm, this novel titanium implant coating with DMADDM was promising in preventing peri-implant disease in an 'ecological manner'. Copyright 2017 by the authors. Licensee MDPI, Basel, Switzerland.
SponsorsThis study was supported by the National Key Research and Development Program of China 2016YFC1102700 (X.Z.); the National Natural Science Foundation of China 81372889 (L.C.), 81430011 (X.Z.), 81600858 (B.R.) and 81372890 (M.F.); the International Science and Technology Cooperation Program of Sichuan Province 2017HH0008 (L.C.); Recruitment Program for Young Professionals (M.F.); the Innovative Research Team Program of Sichuan Province, NIH R01 DE17974 (H.X.) and the University of Maryland School of Dentistry bridge fund (H.X.).
Identifier to cite or link to this itemhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85036570416&doi=10.3390%2fmolecules22112009&partnerID=40&md5=ff9948cf6f8956a1c0eae8a95d3e54e2; http://hdl.handle.net/10713/10008