Heat-polymerized resin containing dimethylaminododecyl methacrylate inhibits Candida albicans biofilm
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AbstractThe prevalence of stomatitis, especially caused by Candida albicans, has highlighted the need of new antifungal denture materials. This study aimed to develop an antifungal heat-curing resin containing quaternary ammonium monomer (dimethylaminododecyl methacrylate, DMADDM), and evaluate its physical performance and antifungal properties. The discs were prepared by incorporating DMADDM into the polymer liquid of a methyl methacrylate-based, heat-polymerizing resin at 0% (control), 5%, 10%, and 20% (w/w). Flexure strength, bond quality, surface charge density, and surface roughness were measured to evaluate the physical properties of resin. The specimens were incubated with C. albicans solution in medium to form biofilms. Then Colony-Forming Units, XTT assay, and scanning electron microscope were used to evaluate antifungal effect of DMADDM-modified resin. DMADDM modified acrylic resin had no effect on the flexural strength, bond quality, and surface roughness, but it increased the surface charge density significantly. Meanwhile, this new resin inhibited the C. albicans biofilm significantly according to the XTT assay and CFU counting. The hyphae in C. albicans biofilm also reduced in DMADDM-containing groups observed by SEM. DMADDM modified acrylic resin was effective in the inhibition of C. albicans biofilm with good physical properties. Copyright 2017 by the authors.
SponsorsThis study was supported by International Science and Technology Cooperation Program of China (2014DFE30180) (XZ), National Natural Science Foundation of China grant 81372889 (LC), 81430011 (XZ), 81372890 (MF), the Youth Grant of the Science and Technology Department of Sichuan Province, China 2017JQ0028 (LC), Innovative Research Team Program of Sichuan Province, Scientific Research Starting Foundation (grant number 2015SCU11015) for Young Teachers of Sichuan University, the National Institutes of Health (R01 DE17974 to H.X.), and a seed grant (H.X.) from the University of Maryland School of Dentistry.
Candida albicans biofilms
Identifier to cite or link to this itemhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85018481677&doi=10.3390%2fma10040431&partnerID=40&md5=a49a5922ddb2fde6203c21665f504373; http://hdl.handle.net/10713/10005