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dc.contributor.authorTolbert, W.D.
dc.contributor.authorGohain, N.
dc.contributor.authorAlsahafi, N.
dc.date.accessioned2019-07-15T16:16:56Z
dc.date.available2019-07-15T16:16:56Z
dc.date.issued2017
dc.identifier.urihttps://www.scopus.com/inward/record.uri?eid=2-s2.0-85031804948&doi=10.1016%2fj.str.2017.09.009&partnerID=40&md5=15a525461688a21e2095988403823741
dc.identifier.urihttp://hdl.handle.net/10713/10001
dc.description.abstractAntibodies can have an impact on HIV-1 infection in multiple ways, including antibody-dependent cellular cytotoxicity (ADCC), a correlate of protection observed in the RV144 vaccine trial. One of the most potent ADCC-inducing epitopes on HIV-1 Env is recognized by the C11 antibody. Here, we present the crystal structure, at 2.9 Å resolution, of the C11-like antibody N12-i3, in a quaternary complex with the HIV-1 gp120, a CD4-mimicking peptide M48U1, and an A32-like antibody, N5-i5. Antibody N12-i3 recognizes an epitope centered on the N-terminal “eighth strand” of a critical β sandwich, which our analysis indicates to be emblematic of a late-entry state, after the gp120 detachment. In prior entry states, this sandwich comprises only seven strands, with the eighth strand instead pairing with a portion of the gp120 C terminus. The conformational gymnastics of HIV-1 gp120 thus includes altered β-strand pairing, possibly to reduce immunogenicity, although nevertheless still recognized by the human immune system. Copyright 2017 Elsevier Ltden_US
dc.description.sponsorshipThis work was supported by NIH grants: NIAID R01 AI116274 to M.P., R01AI129769 to M.P. and A.F., NIGMS R01 GM117836 to K.R., NIAID P01 AI120756 , and the Bill and Melinda Gates Foundation : OPP1033109 to G.K.L. This work was partially supported also by a CIHR foundation grant 352417 to A.F. who is supported by the Canada Research Chairs program .en_US
dc.description.urihttps://www.doi.org/10.1016/j.str.2017.09.009en_US
dc.language.isoen_USen_US
dc.publisherCell Pressen_US
dc.relation.ispartofStructure
dc.subjectantibody-dependent cellular cytotoxicity (ADCC)en_US
dc.subjectC11 epitope regionen_US
dc.subjectC11-like antibodyen_US
dc.subjectcluster A epitopesen_US
dc.subjectcrystal structureen_US
dc.subjectHIV-1 entryen_US
dc.subjectN12-i3 antibodyen_US
dc.titleTargeting the Late Stage of HIV-1 Entry for Antibody-Dependent Cellular Cytotoxicity: Structural Basis for Env Epitopes in the C11 Regionen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.str.2017.09.009
dc.identifier.pmid29056481


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