NMR is a widely used non-invasive and non-destructive analytical method to study the stability of small molecules in different chemical environments. It qualitatively identifies compounds and quantitatively monitors composition and degradation through the evaluation of impurities.1,2 v Potential impurities originate from starting materials, intermediate products, or by- products, and degradation of the original compound and residual solvents, which are detected by changes in peak structure that arise in the spectra.3 v A 400 MHz Varian spectrometer 1H NMR characterizes the behavior of small molecules by analyzing the changes in chemical shift, integration, and splitting of peaks in spectra over time to detect and identify small molecule contaminants.2,3,4,5 v This ongoing study analyzes small molecule samples of selective ALDH-2 inhibitors for non-alcoholic fatty liver disease and Gallium-Salophen complexes as Hem-O inhibitors against Pseudomonas aeruginosa by subjecting them to different media measured at various time points for stability