Pseudomonas aeruginosa is a common bacteria that can cause various infections in the human body and survives in the body during chronic infections via iron from heme. The Wilks Lab is interested in finding ways to disrupt the transportation of heme to P. aeruginosa in order to cure infections. The PhuS protein is part of the heme uptake systems that affect heme delivery into P. aeruginosa and is known to be regulated by heme and iron. This project specifically focuses on finding the heme dependent transciptional regulator of the phuS gene promoter.