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Protocol for Microsatellite Genotyping by Unlinked Markers
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Date
2012-02-17
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University of Maryland School of Medicine. Center for Vaccine Development. Malaria Group
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Other
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"Protocol is suitable for genotyping unlinked P. falciparum microsatellite markers by PCR amplification of genomic DNA extracted from dried blood spots on Whatman 3MM filter paper ..."
Includes the following sections: Introduction, 1.0 Purpose; 2.0 References; 3.0 Materials; 4.0 Equipment; 5.0 Safety and Precautions; 6.0 Procedure; 6.1 Notes; 6.2 PCR set up: Primary and Secondary (Heminested) PCR Reactions; 6.3 Preparation of Sample Plates for Capillary Electrophoresis; 6.4 Reporting Results using GeneMapper® ver 4.0; 7.0 Appendices
Includes the following sections: Introduction, 1.0 Purpose; 2.0 References; 3.0 Materials; 4.0 Equipment; 5.0 Safety and Precautions; 6.0 Procedure; 6.1 Notes; 6.2 PCR set up: Primary and Secondary (Heminested) PCR Reactions; 6.3 Preparation of Sample Plates for Capillary Electrophoresis; 6.4 Reporting Results using GeneMapper® ver 4.0; 7.0 Appendices
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