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PCR Protocol for PfCRT Pyrosequencing
Authors
Advisor
Date
2012-02-09
Embargo until
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Book title
Publisher
University of Maryland School of Medicine. Center for Vaccine Development. Malaria Group
Peer Reviewed
Type
Other
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Other Titles
SUBTITLE: (codons 72, 74, 75, 76, 220,271,326, and 371)
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Abstract
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Description
"Provides a method for performing PCR on genomic DNA from dried blood spots on Whatman 3MM filter paper ... for subsequent genotyping of pfcrt codons ..."
Includes the following sections: 1.0 Purpose; 2.0 References; 3.0 Materials; 4.0 Equipment; 5.0 Safety and Precautions; 6.0 Procedure; 6.1 DNA extraction from blood spotted filter papers; 6.2 Setting up PCR Reactions
Includes the following sections: 1.0 Purpose; 2.0 References; 3.0 Materials; 4.0 Equipment; 5.0 Safety and Precautions; 6.0 Procedure; 6.1 DNA extraction from blood spotted filter papers; 6.2 Setting up PCR Reactions
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Keyword
pfcrt codon 72
pfcrt codon 74
pfcrt codon 75
pfcrt codon 76
pfcrt codon 220
pfcrt codon 271
pfcrt codon 326
pfcrt codon 356
pfcrt codon 371
pyrosequencing
University of Maryland, Baltimore. Center for Vaccine Development (CVD)
Genotyping Techniques--methods
Plasmodium falciparum--genetics
Polymerase Chain Reaction
Sequence Analysis--methods
pfcrt codon 74
pfcrt codon 75
pfcrt codon 76
pfcrt codon 220
pfcrt codon 271
pfcrt codon 326
pfcrt codon 356
pfcrt codon 371
pyrosequencing
University of Maryland, Baltimore. Center for Vaccine Development (CVD)
Genotyping Techniques--methods
Plasmodium falciparum--genetics
Polymerase Chain Reaction
Sequence Analysis--methods