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PCR Protocol for PfCRT Pyrosequencing

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2012-02-09
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University of Maryland School of Medicine. Center for Vaccine Development. Malaria Group
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Other
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SUBTITLE: (codons 72, 74, 75, 76, 220,271,326, and 371)
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"Provides a method for performing PCR on genomic DNA from dried blood spots on Whatman 3MM filter paper ... for subsequent genotyping of pfcrt codons ..."
Includes the following sections: 1.0 Purpose; 2.0 References; 3.0 Materials; 4.0 Equipment; 5.0 Safety and Precautions; 6.0 Procedure; 6.1 DNA extraction from blood spotted filter papers; 6.2 Setting up PCR Reactions
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