Title of Thesis: Comparison of Shear-bond Strength of Orthodontic Brackets on Enamel with use of Pro Seal with Assure Plus David Greene, Master of Biomedical Sciences, 2023 Thesis directed by: Dr. Dina Sanchez, Director of Predoctoral Orthodontics, Clinical Assistant Professor, Department of Orthodontics and Pediatric Dentistry We aim to investigate the shear bond strength of orthodontic brackets bonded with Assure Plus alone and in combination with Pro Seal, following the manufacturer's recommended bonding protocol. Forty-two (42) extracted premolar human teeth were randomly assigned to two bonding groups, the control group (Assure Plus) and the test group (Assure Plus and Pro Seal). After bonding, the teeth were stored at 37°C for 24 hours. Next, the shear bond strength test was completed. The results of the Mann- Whitney test showed there was not a statistical difference of shear bond strength between the two groups for the variables, Peak Load and Shear Bond Strength. The use of Pro Seal with Assure Plus as a preventive measure for white spot lesions during orthodontic treatment does not negatively impact the shear bond strength of orthodontic brackets during treatment.