The purpose of this study is to investigate how the addition of antibacterial materials, Di-methylaminododecyl methacrylate (DMAHDM), and Nano-Silver (NAg) to AH Plus® will affect the mechanical and antibacterial properties of the sealer. Setting time, flow, dimensional change and solubility were evaluated as a set of mechanical tests according to ANSI/ADA specifications. Direct contact test (DCT) was performed to evaluate antibacterial properties at day 1,7 and 14 after mixing against E.faecalis. The DCT was measured with optical density (OD) and colony-forming units (CFU) counts. When 0.15%NAg and 2.5% DMAHDM was incorporated into AH Plus®, there was no significant difference in mechanical properties and improved antibacterial properties through 14 days when compared to AH Plus® alone. This study found that up to 0.15% NAg and 2.5% DMAHDM can be added to AH Plus® without changing the existing mechanical properties of AH Plus® while incorporating long-term antibacterial properties.